Composite

Part:BBa_K3786004:Design

Designed by: Camila Ballenghien   Group: iGEM21_Paris_Bettencourt   (2021-10-21)


TMM expression controlled by the Arabinose promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
    Illegal AgeI site found at 487
    Illegal AgeI site found at 710
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56


Design Notes

The composite part was constructed using CPEC, therefore we needed to ensure that the overhangs designed for TMM and pTS00 would anneal


Source

The TMM coding sequence was cloned into the pTS00 plasmid (from our home institution: Centre de Recherche Interdisciplinaire) using Circular Polymerase Extension cloning (CPEC). The TMM sequence was amplified from the iGEM part BBa_K1403015 and the rest of the parts were found in the pTS00 plasmid.

References